DAAO Active

Alternative Names: D-Amino-Acid Oxidase, D-Amino Acid Oxidase 2, DAMOX, DAAO, EC 1.4.3, EC 1.4.3.3, OXDA.

DAAO is an enzyme that uses FAD as a cofactor to oxidize D-amino acids to their imino acid form, producing ammonia and hydrogen peroxide. It helps maintain acid-base balance in kidney tissue and detoxifies molecules that accumulate as cells age.

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  • Sku: PSB-enz-1142-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human D-Amino Acid Oxidase, BioActive is a protein belonging to the oxidase enzyme family. With a molecular weight of approximately 37 kDa, this enzyme plays a significant role in catalyzing the oxidation of D-amino acids. Its bioactive nature allows for various applications in research and biotechnology, making it a valuable tool in studying amino acid metabolism and related pathways.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
Specific activity is > 3.5unit/mg, in which one unit will oxidatively deaminate 1.0 umole of D-alanine to pyruvateper minute at pH 8.5 at 37C, in the presence of catalase.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation DAAO Human protein (0.5mg/ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 20% glycerol & 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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DAAO Active

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