DAAO

Alternative Names: D-amino-acid oxidase, DAAO, DAO, DAMOX, OXDA, MGC35381.

DAAO is a peroxisomal enzyme that utilizes FAD as a coenzyme to catalyze the oxidation of D-amino acids to their corresponding amino acids, generating ammonia and hydrogen peroxide as byproducts. DAAO shows selectivity towards D-amino acids, with a preference for those with small hydrophobic side chains followed by polar, aromatic, and basic groups. It does not act on naturally occurring L-amino acids or acidic amino acids. DAAO's physiological functions include potential involvement in acid-base balance in the kidney, detoxification of accumulated D-amino acids during aging, and regulation of the neuromodulator D-serine levels in the brain. Additionally, DAAO exhibits high activity towards D-DOPA and is inhibited by creatinine in uremia. While DAAO may play a role in the pathophysiology of schizophrenia, it is not implicated in bipolar disorder.

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  • Sku: PSB-enz-425-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human D-Amino Acid Oxidase is an enzyme that belongs to the oxidase family, specifically targeting D-amino acids. With a molecular weight of approximately 40 kDa, this enzyme plays a significant role in the metabolism of D-amino acids by catalyzing their oxidation. Its recombinant form allows for enhanced stability and activity, making it a valuable tool in various research and biotechnological applications.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 41.6 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The DAAO solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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DAAO
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