CYB5R3

Alternative Names: EC 1.6.2.2, Cytochrome b5 reductase 3, DIA1, B5R, Diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, Diaphorase-1

CYB5R3 is a cytochrome b5 reductase found in both membrane-bound and soluble forms. The membrane-bound form is present in somatic cells, while the soluble form is found in erythrocytes. The membrane-bound form primarily functions in fatty acid modification, cholesterol production, and drug metabolism within the endoplasmic reticulum. The erythrocyte form is responsible for reducing methemoglobin. The membrane-bound form contains both membrane-binding and catalytic domains, while the soluble form only has the catalytic domain. Mutations in this gene lead to methemoglobinemias.

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  • Sku: PSB-pro-1026-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Cytochrome B5 Reductase 3 is an enzyme that plays a vital role in the metabolism of various compounds within the human body. Belonging to the cytochrome B5 reductase family, it acts as an electron donor for cytochrome P450 enzymes, facilitating their enzymatic activities. With a molecular weight of approximately X kDa, this protein is involved in electron transfer reactions, aiding in the reduction of cytochrome B5 and other substrates. Its significance lies in its contribution to the efficient functioning of metabolic processes, making it a subject of interest in biochemical studies and drug development.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 34.0 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The CYB5R3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMFQRST PAITLESPDI KYPLRLIDRE IISHDTRRFR FALPSPQHIL GLPVGQHIYL SARIDGNLVV RPYTPISSDD DKGFVDLVIK VYFKDTHPKF PAGGKMSQYL ESMQIGDTIE FRGPSGLLVY QGKGKFAIRP DKKSNPIIRT VKSVGMIAGG TGITPMLQVI RAIMKDPDDH TVCHLLFANQ TEKDILLRPE LEELRNKHSA RFKLWYTLDR APEAWDYGQG FVNEEMIRDH LPPPEEEPLV LMCGPPPMIQ YACLPNLDHV GHPTERCFVF.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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CYB5R3
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