CTSF, Sf9

Alternative Names: CTSF, CATSF, CLN13.

Cathepsin F (CTSF) belongs to the peptidase C1 family, specifically the papain family cysteine proteinases involved in the lysosomal proteolytic system. The gene for CTSF is found on chromosome 11q13, near the gene for cathepsin W. CTSF is widely expressed in various tissues and plays a role in protein breakdown. It is also implicated in certain degradative processes associated with cancer cell growth, as it is highly expressed in certain cancer cell lines.

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  • Sku: PSB-enz-1167-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Cathepsin-F, Sf9 is a member of the cathepsin family of proteases. This enzyme plays a key role in the degradation of proteins within lysosomes. With a molecular weight of approximately 31 kDa, Cathepsin-F is involved in various physiological processes, including antigen processing and bone resorption. The recombinant form of this enzyme produced in Sf9 insect cells is commonly used in research and drug development for its ability to cleave specific peptide bonds with high specificity and efficiency.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Sf9 - Baculovirus cells
Purity 90.0 by SDS-PAGE
Activity
 Specific activity is > 5 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Phe-ArgAMC to Z-Phe-Arg and AMC per minute at pH 5.0 at 37℃.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 52.5 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation CTSF protein solution (0.25mg/ml) contains Phosphate buffered saline (pH7.4) and 40% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ADLAPAQPRA ASFQAWGPPS PELLAPTRFA LEMFNRGRAA GTRAVLGLVR GRVRRAGQGS LYSLEATLEE PPCNDPMVCR LPVSKKTLLC SFQVLDELGR HVLLRKDCGP VDTKVPGAGE PKSAFTQGSA MISSLSQNHP DNRNETFSSV ISLLNEDPLS QDLPVKMASI FKNFVITYNR TYESKEEARW RLSVFVNNMV RAQKIQALDR GTAQYGVTKF SDLTEEEFRT IYLNTLLRKE PGNKMKQAKS VGDLAPPEWD WRSKGAVTKV KDQGMCGSCW AFSVTGNVEG WFLNQGTLL SLSEQELLDC DKMDKACMGG LPSNAYSAIK NLGGLETEDD YSYQGHMQSC NFSAEKAKVY INDSVELSQN EQKLAAWLAK RGPISVAINA FGMQFYRHGI SRPLRPLCSP WLIDHAVLLV GYGNRSDVPF WAIKNSWGTD WGEKGYYYLH RGSGACGVNT MASSAVVDHH HHHH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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CTSF, Sf9
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