CTLA4, Sf9

Alternative Names: Not Available

CTLA-4 is an immunoglobulin superfamily member that encodes a protein transmitting inhibitory signals to T cells. It consists of a V domain, transmembrane domain, and cytoplasmic tail, with alternate splice variants producing different isoforms. The membrane-bound isoform acts as a homodimer connected by a disulfide bond, while the soluble isoform acts as a monomer. Mutations in this gene are linked to various autoimmune diseases such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, lupus erythematosus, and thyroid-associated orbitopathy.

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  • Sku: PSB-cyt-952-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Cytotoxic T-Lymphocyte Associated Antigen-4, Sf9 is a protein belonging to the immunoglobulin superfamily. With a molecular weight of approximately 40-45 kDa, this protein plays a role in regulating immune responses by inhibiting T-cell activation. Produced in Sf9 insect cells using recombinant DNA technology, this antigen is commonly used in research settings to study immune system function and potential therapeutic applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Baculovirus cells
Purity 90% by SDS-PAGE
Activity
Determined by IL-2 ELISA via Jurkat human acute T cell leukemia cells. The ED50 range ≤ 150 ng/ml with Human B7-1/CD80.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
14.6 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation CTLA4 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ADLKAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI DPEPCPDSDH HHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CTLA4, Sf9

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