CTLA4, IgG-His, Active

Alternative Names: CD, CD152, CTLA4, CTLA-4, Cytotoxic T-Lymphocyte Associated Antigen-4, ALPS5, CELIAC3, GRD4, GSE, IDDM12, igG-His Tag.

The CTLA4 protein inhibits immune response by binding to CD80 or CD86 on antigen presenting cells. In cancers, it can enhance the function of T cells.

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  • Sku: PSB-cyt-1144-10µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Cytotoxic T-Lymphocyte Associated Antigen-4, igG-His Tag, Active is a protein belonging to the immunoglobulin superfamily. This protein plays a key role in regulating immune responses by inhibiting T-cell activation. With a molecular weight of approximately 55-60 kDa, this active form of CTLA-4 is commonly used in research and therapeutic applications for studying immune checkpoint pathways and developing novel immunotherapies.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Baculovirus cells
Purity 95.0 % by SDS-PAGE
Activity
Determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells.  ED50 range for this is ≤ 150 ng/ml with Human B7 1/CD80.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
40.8 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The CTLA4 solution (0.5mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ADLKAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMGNELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVIDPEPCPDSDL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVDVSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSNKALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNGQPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CTLA4, IgG-His, Active

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