CTDSPL

Alternative Names: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3, CTD small phosphatase-like protein, CTDSP-like, C3orf8, NIF-like protein, Nuclear LIM interactor-interacting factor 1, NLI-interacting factor 1, Protein YA22, hYA22, RBSP3, Small C-terminal domain phosphatase 3, SCP3, Small CTD phosphatase 3, CTDSPL, NIF1, NIFL, SCP3, YA22, PSR1.

CTD small phosphatase-like protein (CTDSPL) is known for its ability to dephosphorylate 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of POLR2A, the largest subunit of RNA polymerase II. It negatively regulates RNA polymerase II transcription by promoting the transition from initiation/capping to processive transcript elongation. CTDSPL is utilized by REST to silence neuronal genes in non-neuronal cells that contain RE-1 elements.

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  • Sku: PSB-enz-569-5µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human CTD Small Phosphatase-Like is a member of the small C-terminal domain phosphatase (SCP) family. This protein belongs to the SCP-like phosphatase superfamily and plays a role in the dephosphorylation of the C-terminal domain of RNA polymerase II. Recombinant Human CTD Small Phosphatase-Like has a molecular weight of approximately 18 kDa and is involved in regulating transcriptional activity in eukaryotic cells. This protein is commonly used in research settings to study the mechanisms of gene expression and transcriptional regulation.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 23.9 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The CTDSPL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMKYLLP EVTVLDYGKK CVVIDLDETL VHSSFKPISN ADFIVPVEID GTIHQVYVLK RPHVDEFLQR MGQLFECVLF TASLAKYADP VADLLDRWGV FRARLFRESC VFHRGNYVKD LSRLGRELSK VIIVDNSPAS YIFHPENAVP VQSWFDDMTD TELLDLIPFF EGLSREDDVY SMLHRLCNR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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CTDSPL
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