CSTF1, Sf9

Alternative Names: Cleavage Stimulation Factor Subunit 1, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, 50kD, Cleavage Stimulation Factor 50 KDa Subunit, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, 50kDa, Cleavage Stimulation Factor, 3 Pre-RNA, Subunit 1, CstFp50, CF-1 50 KDa Subunit, CSTF 50 KDa Subunit, CstF-50.

Cleavage stimulation factor subunit 1 (CSTF1) is a component involved in the process of polyadenylation and 3'end cleavage of pre-mRNAs. CSTF1 is one of the subunits that form cleavage stimulation factor (CSTF), similar to the mammalian G protein beta subunits. This subunit contains transducin-like repeats. CSTF1 plays a crucial role in the polyadenylation and 3'-end cleavage of mammalian pre-mRNAs by facilitating the interaction of CSTF with other factors, resulting in the formation of a stable complex on the pre-mRNA.

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  • Sku: PSB-pro-2436-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Cleavage Stimulation Factor 1, Sf9, is a protein that belongs to the family of cleavage stimulation factors. With a molecular weight of [insert weight], this protein plays a significant role in various cellular processes. It functions as a key regulator of mRNA 3' end processing, facilitating the efficient cleavage and polyadenylation of pre-mRNAs. By binding to the consensus sequence elements in the pre-mRNA, Sf9 ensures the accurate formation of mature mRNA transcripts. This recombinant protein offers researchers a valuable tool for studying the intricate mechanisms underlying mRNA processing and gene expression regulation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Baculovirus cells
Purity 90.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation CSTF1 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4), 1mM DTT and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CSTF1, Sf9

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