CSTA Active

Alternative Names: Cystatin-A, Cystatin-AS, CSTA, Stefin-A, STFA, STF1.

Human cystatin A, also known as CSTA or Stefin A, is a member of family 1 of the cystatin superfamily. This family is distinguished by its lack of disulfide bonds and carbohydrates. CSTA acts as an intracellular inhibitor, controlling the function of cysteine proteases from the papain family, including Cathepsins B, H and L. CSTA has been linked to various disease conditions, potentially due to its involvement in altered proteolytic processes during cancer development.

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  • Sku: PSB-pro-086-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Cystatin-A, Active is a proteinaceous inhibitor primarily involved in regulating protease activity. Belonging to the cystatin family, this molecule plays a crucial role in modulating the activity of cysteine proteases, thus maintaining cellular homeostasis. With a molecular weight of approximately X kDa, Recombinant Human Cystatin-A, Active exhibits potent inhibitory effects on target enzymes, making it a valuable tool in various research applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
The IC50 value is < 1.0nM. The inhibitory function of CSTA on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Cystatin-A (1mg/ml) in 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CSTA Active

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