CST3, His Active

Alternative Names: Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.

Cystatin C is a member of the cystatin protein family, which includes proteins with various cystatin-like sequences. Some of these proteins can inhibit cysteine proteases, while others cannot. The cystatin protein family includes type 1 cystatins, type 2 cystatins, and kininogens. The type 2 cystatin genes are mostly located on chromosome twenty. Cystatin C, the most common extracellular inhibitor of cysteine proteases, is found in the cystatin locus. It is present in large quantities in biological fluids and is present in all tissues of the body.

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  • Sku: PSB-pro-2632-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Cystatin C, His BioActive is a protein that is part of the cystatin family. With a molecular weight of approximately X kilodaltons, it plays a significant role in regulating the activity of cysteine proteases. This bioactive protein is produced through recombinant DNA technology and contains a histidine tag for easy purification and detection. Its precise structure and function make it a valuable tool in various research applications, including enzymatic studies and drug development.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95.0 by SDS-PAGE
Activity
The IC50 value is < 2.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25˚C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
15.6 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The CST3 solution (0.25mg/1ml) contains 0.1M NaCl, 20mM Tris-HCl buffer (pH 8.0) and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSSPGKPPRL VGGPMDASVE EEGVRRALDF AVGEYNKASN DMYHSRALQV VRARKQIVAG VNYFLDVELG RTTCTKTQPN LDNCPFHDQP HLKRKAFCSF QIYAVPWQGT MTLSKSTCQD A

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CST3, His Active

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