CRKL

Alternative Names: CRKL, Crk-like protein.

CRKL is a protein kinase with SH2 and SH3 domains, through which it activates the RAS and JUN kinase signaling pathways. It transforms fibroblasts in a RAS-dependent way. CRKL can serve as a substitute for the BCR-ABL tyrosine kinase and is involved in fibroblast transformation by BCR-ABL. Additionally, it possesses oncogenic potential.

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  • Sku: PSB-pro-063-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human V-crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like is a protein that belongs to the family of avian-like sarcoma viruses. With a weight of approximately [insert weight], this protein plays a significant role in various cellular processes. Its functional importance lies in its ability to regulate cell growth, differentiation, and transformation. By interacting with various signaling molecules, this protein modulates intracellular pathways involved in cell proliferation and survival. Understanding the role of Recombinant Human V-crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like is crucial for unraveling the intricate mechanisms underlying oncogenesis and potential therapeutic interventions.

Current Lead Time

7-14 Days

Product Specifications

Species
Avian
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
35.9 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The CRKL solution (1 mg/ml) 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRDSSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD ENE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CRKL

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