COX5B

Alternative Names: Mitochondrial, Cytochrome c oxidase polypeptide Vb, COXVB, COX5B, Cytochrome c oxidase subunit 5B, mitochondrial precursor.

Cytochrome c oxidase subunit VB (COX5B) is a vital enzyme in the mitochondrial respiratory chain. It is part of a complex that transfers electrons from cytochrome c to molecular oxygen, thereby generating a proton electrochemical gradient across the inner mitochondrial membrane. There are two isoforms of COX5: COX5a and COX5b. COX5A is up-regulated during high cellular respiration when oxygen levels are adequate. On the other hand, COX5B transcription increases during low oxygen levels, ensuring efficient turnover of the COX apoenzyme.

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  • Sku: PSB-pro-1513-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Cytochrome C Oxidase Subunit Vb is a protein that plays a significant role in the respiratory chain, particularly in the final step of oxidative phosphorylation. Belonging to the cytochrome c oxidase (COX) family, it acts as a crucial subunit in the assembly and stabilization of the COX complex. With a molecular weight of approximately X kDa, this protein contributes to the catalytic activity of COX, facilitating the transfer of electrons from cytochrome c to molecular oxygen. Its functional significance in cellular respiration makes it a subject of interest in various scientific studies.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation COX5B protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 20% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSASGGGVP TDEEQATGLE REIMLAAKKG LDPYNVLAPK GASGTREDPN LVPSISNKRI VGCICEEDNT SVVWFWLHKG EAQRCPRCGA HYKLVPQQLA H.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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COX5B

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