COMT

Alternative Names: Catechol O-methyltransferase, COMT, EC 2.1.1.6

COMT is an enzyme involved in the transfer of a methyl group to catechol substrates, such as neurotransmitters, leading to the degradation of catecholamine transmitters. It is located in postsynaptic neurons and plays a role in metabolizing catechol estrogen drugs used in treating various conditions. COMT exists in two forms, soluble and membrane-bound, with differences in their N-termini. Inhibitors of COMT can increase its availability and are used in treating patients with Parkinson's disease.

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  • Sku: PSB-enz-400-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Catechol-O-Methyltransferase (COMT) is an enzyme belonging to the methyltransferase family, responsible for the transfer of a methyl group from S-adenosyl-L-methionine to catecholamines such as dopamine, norepinephrine, and epinephrine. This enzyme plays a critical role in the regulation of neurotransmitter levels in the brain. With a molecular weight of approximately 30 kDa, Recombinant Human COMT is widely used in research and drug development for its involvement in various neurological disorders.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation COMT protein in 20mM Tris-HCl buffer, pH-8, 1mM MgCl2 and 10% Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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COMT

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