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COL4A3, Biotin

Alternative Names: Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.

Type IV collagen, a crucial component of basement membranes, consists of three alpha subunits encoded by six different genes (alpha 1 through alpha 6). These subunits can combine to form a triple helix structure. In Goodpasture syndrome, autoantibodies target collagen molecules in alveoli and glomeruli basement membranes, specifically binding to the non-collagenous C-terminal domain of the protein. Unique alternative transcripts are found in the human COL4A3 gene, showing limited alternative splicing in the six exons that encode this C-terminal domain. COL4A3 is also associated with the autosomal recessive form of Alport syndrome, with mutations occurring in the exons that encode the C-terminal region. COL4A3 is organized in a head-to-head arrangement with another type IV collagen gene, both sharing a common promoter. Additionally, some exons of COL4A3 are interspersed with exons of an unidentified gene on the opposite strand.

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  • Sku: PSB-pro-2564-50µg
  • Vendor: ProSpec-Tany
$600.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Collagen Type IV Alpha 3, Biotinylated is a modified form of collagen protein that has been biotinylated for enhanced detection and binding capabilities. Belonging to the collagen family, this protein is an important component of the extracellular matrix, providing structural support and stability to various tissues and organs. With a molecular weight of approximately X kDa, this biotinylated variant offers a valuable tool for research applications, allowing for precise localization and visualization of collagen Type IV Alpha 3 in biological samples.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Sf9 - Insect cells
Purity 87 by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homotrimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation COL4A3 Human biotin contains 20mM HEPES, pH 7.5, 0.02% SDS and 0.01mM EDTA.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

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