COL4A3

Alternative Names: Goodpasture antigen, Collagen alpha-3(IV) chain, COL4A3, Glomerular Basal Membrane, GBM.

Type IV collagen, a crucial component of basement membranes, is composed of 3 alpha subunits encoded by 6 different genes. These alpha subunits combine with one another to form the triple helix structure of type IV collagen. The Goodpasture syndrome occurs when autoantibodies attach to collagen molecules in the basement membranes of alveoli and glomeruli. These autoantibodies primarily target the non-collagenous C-terminal domain of the protein. Unique alternative transcripts have been observed in the human COL4A3 gene, specifically within the six exons that encode the C-terminal domain. Mutations in COL4A3 are also associated with an autosomal recessive form of Alport syndrome, with these mutations also present within the exons encoding the C-terminal region. COL4A3 is positioned in a head-to-head arrangement with another type IV collagen gene, sharing a common promoter. Additionally, some exons of COL4A3 are interspersed with exons of an unknown gene on the opposite strand.

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  • Sku: PSB-pro-387-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Collagen Type IV Alpha 3 is a protein that plays a significant role in maintaining the structural integrity of basement membranes. Belonging to the collagen family, it is a vital component of the extracellular matrix, providing support and stability to various tissues and organs. With a molecular weight of approximately X kDa, this protein is involved in cell adhesion, migration, and differentiation processes. The Recombinant Human Collagen Type IV Alpha 3 protein is widely studied due to its relevance in various physiological and pathological conditions, making it an essential subject of scientific investigation.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Sf9 - Insect cells
Purity 95.0 % by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homomultimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The protein solution contains 20mM Hepes, pH 8.0 and 4M Urea.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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COL4A3
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