CMBL

Alternative Names: JS-1, Carboxymethylenebutenolidase homolog, CMBL

Carboxymethylenebutenolidase homolog (CMBL) is a cysteine hydrolase belonging to the dienelactone hydrolase family, primarily found in the liver cytosol. CMBL is the human equivalent of Pseudomonas dienelactone hydrolase, an enzyme involved in bacterial halocatechol degradation. CMBL's main function is to activate medoxomil-ester prodrugs containing cyclic esters with an oxygen atom, by preferentially cleaving them. The enzyme is sensitive to inhibition by PCMB (p-chloromercuribenzoate), and its gene is located on human chromosome 5p15.2. CMBL is also capable of activating beta-lactam antibiotics like faropenem medoxomil and lenampicillin. It is widely distributed in the body, being most abundant in the liver, followed by the kidney, small intestine, and colon.

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  • Sku: PSB-enz-634-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Carboxymethylenebutenolidase is an enzyme belonging to the hydrolase family that plays a role in the metabolism of certain compounds. With a molecular weight of approximately 30 kDa, this enzyme catalyzes the hydrolysis of carboxymethylenebutenolidase substrates, leading to the formation of specific products. Its function is essential for the breakdown of these compounds in various biological processes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 30.6 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The CMBL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM EDTA.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMANEAY PCPCDIGHRL EYGGLGREVQ VEHIKAYVTK SPVDAGKAVI VIQDIFGWQL PNTRYIADMI SGNGYTTIVP DFFVGQEPWD PSGDWSIFPE WLKTRNAQKI DREISAILKY LKQQCHAQKI GIVGFCWGGT AVHHLMMKYS EFRAGVSVYG IVKDSEDIYN LKNPTLFIFA ENDVVIPLKD VSLLTQKLKE HCKVEYQIKT FSGQTHGFVH RKREDCSPAD KPYIDEARRN LIEWLNKYM.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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CMBL
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