CKMB Type-2

Alternative Names: CKMB, CKMBI, CKMBITII, Creatine Kinase MB Isoenzyme Type-II

CK-MB Type II has a lysine amino acid at its carboxy-terminal end. In the case of a myocardial infarct or heart attack, damaged heart muscle releases CK-MB Type II into the bloodstream. This results in the cleavage of the C-terminal lysine, leading to the creation of CK-MB Type I. This distinction can be utilized for diagnosing a myocardial infarct.

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  • Sku: PSB-cki-270-10µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Creatine Kinase MB Isoenzyme Type-2 is a protein belonging to the creatine kinase family. With a weight of approximately 84 kDa, this enzyme plays a significant role in energy metabolism by catalyzing the reversible transfer of a phosphate group between creatine and adenosine triphosphate (ATP). This isoform, predominantly found in cardiac muscle, serves as a biomarker for myocardial infarction due to its elevated levels in the bloodstream following cardiac injury. Understanding the properties and functions of Recombinant Human Creatine Kinase MB Isoenzyme Type-2 contributes to the advancement of diagnostic and therapeutic approaches in cardiovascular medicine.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Pichia Pastoris
Purity
Activity
The enzyme activity measured by kinetic assay at 340nm was 650 IU/mg at 37 degrees.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Each mg of protein contains 10mM Tris-HCl, pH-6.8, 0.5mM EDTA and 0.5mM DTT, 50% (v/v) glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CKMB Type-2

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