CKM Type-1

Alternative Names: EC 2.7.3.2, Creatine kinase M-type, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.

Creatine Kinase MM is a cytoplasmic enzyme that plays a role in maintaining energy balance and serves as a significant indicator of heart attacks. It facilitates the transfer of phosphate between ATP and other phosphogens, such as creatine phosphate. In striated muscle and various tissues, it functions as a homodimer, and in the heart, it forms a heterodimer with a brain isozyme. It belongs to the ATP:guanido phosphotransferase protein family.

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  • Sku: PSB-cki-271-10µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Creatine Kinase Muscle Type-1 is an enzyme that plays a significant role in the regulation of energy metabolism within muscle tissues. Belonging to the creatine kinase family, this protein is responsible for the reversible transfer of a phosphate group between creatine and adenosine triphosphate (ATP), facilitating the rapid regeneration of ATP during intense muscle activity. With a molecular weight of approximately 43 kDa, Recombinant Human Creatine Kinase Muscle Type-1 serves as a vital component in maintaining optimal muscle function and energy homeostasis.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Pichia Pastoris
Purity 95% by SDS-PAGE
Activity
 The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml. The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation CKMT1 contains 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, pH 7.2.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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CKM Type-1
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