CKM - Human

Alternative Names: Creatine kinase M-type, Creatine kinase M chain, CKM, M-CK, EC 2.7.3.2, CKMM.

The three isoenzymes (MM, MB, and BB) of Creatine Kinases are present in muscle, cardiac, and brain tissues. These recombinant proteins have great utility in calibrating diagnostic instruments and studying neuromuscular disorders. They can be employed in various neuromuscular applications, including cardiac disease, mitochondrial disorders, myopathies, myasthenia, and many others that result in abnormal levels of Creatine Kinases.

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  • Sku: PSB-cki-273-50,000U
  • Vendor: ProSpec-Tany
$200.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Human Creatine Kinase Muscle, also known as CK-MM, is an enzyme that plays a crucial role in the energy metabolism of skeletal muscle. Belonging to the creatine kinase family, CK-MM is responsible for catalyzing the reversible transfer of a phosphate group between creatine and adenosine triphosphate (ATP), facilitating the rapid regeneration of ATP during intense muscle contraction. With a weight of approximately 43 kDa, this isoform of creatine kinase is specifically found in skeletal muscle tissue, making it a valuable marker for muscle-related diseases and disorders. Its precise measurement and analysis are of great significance in clinical diagnostics and research studies related to muscular pathologies.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human.
Expression System Human Cardiac Tissue
Purity 10.0 % by SDS-PAGE
Activity
 1 unit will transfer 1 µmole of phosphate from Creatine phosphate to ATP/minute at 37 degrees Celsius. Measured at 340nm as one equimolar amount of NADH produced by a coupled reaction. The specific activity was measured and found to be > 100U/mg.
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
 N/A
Structure Monomer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Lyophilized
Formulation The CKM protein was lyophilized from 40mM Tris-HCL, 1mM EDTA, pH 7.5 & 10mM n-Acetyl cysteine.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Lyophilized CKMM although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution IL2 should be stored at 4° C between 2-7 days and for future use below -18° C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Sample Prep Instruction

It is recommended to reconstitute the lyophilized CKMM in sterile distilled water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

References

Not Available
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CKM - Human
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