CKB Pichia

Alternative Names: EC 2.7.3.2, Creatine kinase B-type, Creatine kinase B chain, B-CK, CKB, CKBB, CKBBI.

Creatine Kinase BB is an enzyme found in the cytoplasm that plays a role in maintaining energy balance. This enzyme facilitates the transfer of phosphate groups between ATP and other molecules such as creatine phosphate. In the brain and other tissues, it exists as a homodimer, while in the heart it forms a heterodimer with a closely related muscle enzyme. This enzyme belongs to the ATP:guanido phosphotransferase protein family. A non-functional version of this gene has also been identified.

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  • Sku: PSB-cki-268-10µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Creatine Kinase Brain, Pichia is a protein derived from the Pichia pastoris yeast expression system. Belonging to the family of kinases, this enzyme plays a significant role in cellular energy metabolism. With a molecular weight of approximately 43 kDa, Recombinant Human Creatine Kinase Brain, Pichia is involved in the reversible transfer of phosphate groups between creatine and ATP, facilitating the maintenance of cellular energy homeostasis. Its application spans various fields, including research, diagnostics, and pharmaceutical development.

Current Lead Time

7-14 Days

Product Specifications

Species
 Pichia
Expression System Pichia Pastoris
Purity 95% by SDS-PAGE
Activity
 The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 854 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml. The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 854 IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,171ng/ml.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation CKBB Human contains 10Mm Bis-Tris-HCl pH-6.0, 50% glycerol, 0.5mM EDTA and 0.5mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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CKB Pichia
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