CHST3

Alternative Names: Carbohydrate sulfotransferase 3, CHST3, CHST-3, Chondroitin 6-O-sulfotransferase 1, C6ST-1, C6ST1HSD, Chondroitin 6-sulfotransferase, GST-0.

Carbohydrate Sulfotransferase 3 (CHST3) is a member of the sulfotransferase 1 family, a group of 14 Golgi-localized enzymes that transfer sulfate to specific positions on carbohydrates. CHST3 primarily catalyzes the transfer of sulfate to position 6 of the N-acetylgalactosamine residue of chondroitin, but can also sulfate other residues in keratan sulfate and sialyl LacNAc oligosaccharides. CHST3 is found in the heart, placenta, skeletal muscle, and pancreas, and plays a role in maintaining naive T-lymphocytes in the spleen.

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  • Sku: PSB-enz-1166-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Carbohydrate Sulfotransferase 3 is an enzyme belonging to the sulfotransferase family, specifically involved in the sulfation of carbohydrates. With a molecular weight of approximately 50 kDa, this enzyme plays a key role in the modification of glycosaminoglycans and proteoglycans through the transfer of sulfate groups. Recombinant Human Carbohydrate Sulfotransferase 3 is commonly utilized in research settings for its ability to catalyze the sulfation of carbohydrates for various biological studies.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Sf9 - Baculovirus cells
Purity 90.0 by SDS-PAGE
Activity
 Specific activity is greater than 1,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to Chondroitin Sulfate per minute at pH 7.5, at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 51.3 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation CHST3 protein solution (0.25mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

ADLEKENKII SRVSDKLKQI PQALADANST DPALILAENA SLLSLSELDS AFSQLQSRLR NLSLQLGVEP AMEAAGEEEE EQRKEEEPPR PAVAGPRRHV LLMATTRTGS SFVGEFFNQQ GNIFYLFEPL WHIERTVSFE PGGANAAGSA LVYRDVLKQL FLCDLYVLEH FITPLPEDHL TQFMFRRGSS RSLCEDPVCT PFVKKVFEKY HCKNRRCGPL NVTLAAEACR RKEHMALKAV RIRQLEFLQP LAEDPRLDLR VIQLVRDPRA VLASRMVAFA GKYKTWKKWL DDEGQDGLRE EEVQRLRGNC ESIRLSAELG LRQPAWLRGR YMLVRYEDVA RGPLQKAREM YRFAGIPLTP QVEDWIQKNT QAAHDGSGIY STQKNSSEQF EKWRFSMPFK LAQVVQAACG PAMRLFGYKL ARDAAALTNR SVSLLEERGT FWVTHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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CHST3
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