Chitinase

Alternative Names: Not Available

Chitinase is an enzyme that breaks down glycosidic bonds in chitin, a component of fungi cell walls and some animals' exoskeletons. Organisms that need to remake their own chitin or digest it from fungi or animals have chitinases. These organisms include bacteria like Aeromonas, Bacillus, and Vibrio, some of which can be harmful or feed on detritus. Chitinase expression in organisms is regulated by the NPR1 gene and the salicylic acid pathway, which help resist fungal and insect attacks. Human chitinases are found in gastric juices and likely function as digestive enzymes. Chitinase activity has been identified in the bloodstream and cartilage of humans. Allergies, especially asthma, have been associated with increased chitinase expression, as well as exposure to dust mites and mold spores, which are covered in chitin.

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  • Sku: PSB-enz-031-20µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Clostridium Paraputrificum Chitinase is an enzymatic protein derived from the bacteria Clostridium Paraputrificum. Belonging to the family of chitinases, this protein plays a significant role in the breakdown of chitin, a complex polysaccharide found in the exoskeletons of crustaceans and insects. With a molecular weight of approximately [insert weight], this chitinase exhibits potential applications in various industries, including agriculture, food processing, and waste management. Its enzymatic activity enables the degradation of chitin into simpler compounds, offering potential benefits for biotechnological and environmental processes.

Current Lead Time

7-14 Days

Product Specifications

Species
Clostridium
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
64.2 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Lyophilized
Formulation Chitinase lyophilized from a 0.2µm filtered concentrated solution in PBS.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MRGSGSHHHH HHMYYGDWSI WGGQGNFYPK DIPADKLTHL NFAFMDFNSS GELIYCDKDA AIGHPLGNLG VTYGDVNGGI LNAFQVLKSE NPNLKIGVSL GGWSKSGDFS TIAATPSIRA KFVENVMKFI KYTNMDFVDI DWEYPGDYRE PDKTDNINDE GTPNASAGDK ENYILLLQDL KEALNKQGKE LGKVYELSVA LPAGVSKIEK GIDVDKLFNI VDFANIMTYD MAGAWSTTSG HQTALYTNPN APEEYKGLSV DESVKYYISQ GAEREKIVVG AAYYTRGWEQ VSDKGTDPNN PGLFGEAAVV NKDADLSPTP GALNEAPMKN GEGGRAGGVW GYNALDKLKS KYTGLKEYWD DSAKAPYLYN SETGAFFTYD NIRSIQEKAK YVKENNLGGI IGWMASQDAT TNSTKRDELT TATKESLFGK EDLPKYEIKY TENDITCTVT PVKQSWGSGG VLKMSITNNE KLDESGEVLS TVETSAKTVK NMKVYIKTDG IAITGSQYPA GPVTKEGDYY VIDFGKISDG KLMKAGITFT FDLNLDKAIE DTNNIISIEV SQRMYQTSPE FNRQTIWENT NS.

Storage Instruction

Lyophilized Chitinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Chitinase should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Sample Prep Instruction

It is recommended to reconstitute the lyophilized Chitinase in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

References

Not Available

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Chitinase

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