CHD4

Alternative Names: EC 3.6.4.12, EC 3.6.1, Chromodomain Helicase DNA binding protein 4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi-2b, Mi2-BETA, CHD-4

CHD4 is part of a family of chromodomain helicase-DNA-binding proteins. It is a component of the nucleosome transformation and deacetylase complex involved in transcription regulation. In idiopathic inflammatory myopathies (IIM), the presence of autoantibodies that target CHD4 is a characteristic feature. Mi-2 antibodies, which target CHD4, have a diagnostic sensitivity and specificity of approximately 4-18% and 98-100%, respectively. These antibodies are strongly associated with dermatomyositis and have a high positive predictive value for this particular subset of the disease. They are the only myositis-specific autoantibodies that specifically target the nucleus. It is worth noting that the frequency of Mi-2 antibodies in children is similar to that in adults.

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  • Sku: PSB-pro-112-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Chromodomain Helicase DNA Binding Protein 4 (CHD4) is a member of the CHD family of proteins that play critical roles in chromatin remodeling and gene expression regulation. With a molecular weight of approximately 200 kDa, CHD4 is involved in various cellular processes, including transcriptional repression, DNA repair, and cell cycle progression. This protein is characterized by its ATP-dependent helicase activity and its ability to bind to DNA through its chromodomain. Understanding the functions and mechanisms of CHD4 is crucial for unraveling the intricate dynamics of chromatin structure and gene regulation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Insect cells
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation CHD4 is supplied in 20mM HEPES buffer pH-8.0 and 500mM NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CHD4

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