CFL2

Alternative Names: Cofilin-2, Cofilin- muscle isoform, CFL2, NEM7

CFL2 is an actin-binding protein that belongs to the ADF family and has one ADF-H domain. It is involved in modulating actin within cells by binding to and depolymerizing filamentous F-actin and inhibiting the polymerization of monomeric G-actin. This process is pH-dependent. Mutations in the CFL2 gene are responsible for causing nemaline myopathy type 7 (NEM7).

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  • Sku: PSB-pro-912-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Cofilin-2 is a protein that belongs to the actin-binding family, specifically the cofilin subfamily. With a molecular weight of approximately 18 kilodaltons, this protein plays a significant role in regulating actin dynamics within cells. By severing and depolymerizing actin filaments, Cofilin-2 contributes to cellular processes such as cell migration, cytoskeletal rearrangement, and cell division. Its precise functions and interactions make it a valuable target for research in various fields, including cell biology and cancer biology.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
20.9 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation CFL2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 50mM NaCl.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CFL2

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