CDK2 sf9

Alternative Names: Not Available

Cyclin-dependent kinase 2 (CDK2) is a member of the Ser/Thr protein kinase family and is similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. CDK2 is an essential catalytic subunit of the cyclin-dependent protein kinase complex that specifically operates during the G1-S phase transition of the cell cycle. CDK2 interacts with and is controlled by various regulatory subunits, including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Additionally, CDK2 activity is subject to regulation through protein phosphorylation.

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  • Sku: PSB-pka-066-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human cyclin-dependent kinase 2, sf9, is a protein that belongs to the cyclin-dependent kinase (CDK) family. With a weight of approximately X kDa, this enzyme plays a significant role in regulating cell cycle progression. CDK2, sf9, is involved in phosphorylation events that control the transition between different phases of the cell cycle. This recombinant protein is widely used in scientific research to investigate the mechanisms underlying cell division and proliferation.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Sf9 - Baculovirus cells
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
34.9 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation CDK2 protein solution (0. 5mg/ml) contains Phosphate buffered saline (pH7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRLLE HHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CDK2 sf9

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