CDC42

Alternative Names: CDC42, G25K, G25K GTP-binding protein, CDC42Hs, Cell division control protein 42 homolog

CDC42 is a small GTPase that plays a role in regulating various cellular functions including cell morphology, migration, endocytosis, and cell cycle progression. It is found in the plasma membrane and switches between an active GTP-bound state and an inactive GDP-bound state. In its active state, CDC42 interacts with different proteins to control cellular responses. It is involved in epithelial cell polarization and promotes the formation of thin, actin-rich surface projections known as filopodia. CDC42 also regulates actin polymerization through its interaction with N-WASP, which activates the Arp2/3 complex. Loss of CDC42 function leads to increased uptake of apical proteins during endocytosis and disrupts apical polarity factors like Crumbs. The oncogene Dbl has been identified as a catalyst for the release of GDP from CDC42 protein.

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  • Sku: PSB-pro-727 -10µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Cell Division Cycle 42 (CDC42) is a protein that plays a crucial role in regulating various cellular processes, including cell division and cytoskeletal organization. Belonging to the Rho GTPase family, CDC42 acts as a molecular switch, cycling between an inactive GDP-bound state and an active GTP-bound state. With a molecular weight of approximately 21 kDa, CDC42 serves as a key mediator in signal transduction pathways, influencing cell polarity, migration, and proliferation. Its intricate involvement in multiple cellular functions makes CDC42 a subject of extensive research in understanding fundamental biological processes.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 22.4 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The CDC42 solution contains 20mM Tris-HCl buffer (pH8.0), 1mM DTT, 10% glycerol and 2mM EDTA.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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CDC42
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