CDC34, His

Alternative Names: UB2R1, UBE2R1, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, E2-CDC34, CDC-34, CDC34, Ubiquitin-protein ligase R1, UBC3, EC 6.3.2.19.

CDC34 plays a crucial role in cell cycle control and DNA replication by partnering with various E3 complexes like SCF to target a variety of substrates for ubiquitination and degradation during cell division, signal transduction, and development. Some characterized substrates include I?B, B-Myb, Wee1, MyoD, ICERII?, ATF5, p27Xic1, and p27Kip1. Additionally, substrates like ?-catenin, p21Cip1, E2F, cyclin E, and cyclin D are potential targets of CDC34 due to their reliance on SCF for breakdown. CDC34 has the ability to self-associate via a domain in the C-terminus, and undergoes phosphorylation and ubiquitination in vivo. This protein is valuable for in vitro ubiquitination experiments.

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  • Sku: PSB-enz-355-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Product Details

Product Description

Recombinant Human Cell Division Cycle 34, His Tag is a protein belonging to the cell division cycle family. It plays a role in regulating the cell cycle and promoting cell division. This protein has a molecular weight of approximately 34 kDa and is commonly used in research and biotechnology applications for its ability to interact with other proteins involved in cell division processes. Its His Tag allows for easy purification and detection in experimental settings.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The CDC34 (1mg/ml) protein solution contains 20mM Tris-Hcl pH-8, 10% glycerol, 50mM NaCl and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGTEES.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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CDC34, His
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