CDC34

Alternative Names: UBC3, CDC34, EC 6.3.2.19, CDC-34, UBE2R1, UB2R1, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin-protein ligase R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, E2-CDC34.

CDC34 plays a key role in regulating the cell cycle and DNA replication by targeting various substrates for ubiquitination and degradation in processes such as cell division and signal transduction. Some known substrates of CDC34 include B-Myb, Wee1, MyoD, ATF5, p27Xic1, and p27Kip1. Additionally, proteins like p21Cip1, E2F, cyclin E, and cyclin D are also likely targeted by CDC34. CDC34 can self-associate and is phosphorylated and ubiquitinated in vivo. It is often used in in vitro ubiquitinylation reactions.

  •
  • Sku: PSB-enz-1103-50µg
  • Vendor: ProSpec-Tany
$400.00 USD
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Cell Division Cycle 34, also known as CDC34, is a member of the E2 ubiquitin-conjugating enzyme family. This protein plays a key role in the ubiquitin-proteasome pathway, specifically in the tagging of proteins for degradation. CDC34 has a molecular weight of approximately 22 kDa and is essential for the regulation of cell cycle progression and DNA repair. Its function is critical for maintaining cellular homeostasis and proper protein turnover.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation CDC34 is supplied as a 0.2 μm filtered solution conteining 50mM HEPES, pH 7.0, 10 % Glycerol, 125mM NaCl, , 5 % Trehalose and 1 mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGTEES.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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CDC34
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