CDA

Alternative Names: Cytidine deaminase, Cytidine aminohydrolase, CDA, CDD

Cytidine deaminase (CDA) is an enzyme involved in UMP synthesis by scavenging cytidine and 2'-deoxycytidine. It is crucial for maintaining the cellular pyrimidine pool and can also deactivate chemotherapeutic cytosine nucleoside analogs like Ara-C and 5-azacytidine. CDA is typically found in granulocytes and can form homotetramers. Mutations in the CDA gene have been linked to reduced sensitivity to cytosine arabinoside, a treatment for childhood leukemias.

Skip to product information
  • Sku: PSB-enz-007-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
 per 
Shipping calculated at checkout.

Contact us directly for international purchases.

Title

Product Details

Product Description

Recombinant Human Cytidine Deaminase is an enzyme that belongs to the deaminase family, specifically the cytidine deaminase subfamily. With a molecular weight of approximately 30 kDa, this enzyme plays a crucial role in the metabolism of nucleic acids by catalyzing the deamination of cytidine to uridine. Recombinant Human Cytidine Deaminase is commonly used in biotechnology and pharmaceutical research for its ability to modify nucleic acids for various applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Specific activity is > 10,000pmol/min/ug, and is defined as the amount of required to deaminate 1.0pmole of cytidine per min at pH 7.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
18.3 kDa
Structure Homotetramer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The CDA solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0), 1mM DTT, 2mM EDTA, 100mM NaCl and 40% Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

View full details

CDA

SUBHEADING

Recently viewed products