CASP3, Sf9

Alternative Names: CASP3, CASP-3, CPP32, CPP-32, CPP32B, SCA-1, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1.

CASP3 is a member of the caspase family of cysteine proteases, which are essential for the cell apoptosis process. Inactive proenzymes of caspases are activated through proteolytic processing to generate active enzymes. CASP3 cleaves and activates other caspases like 6, 7, and 9, and is also involved in the cleavage of amyloid-beta 4A precursor protein, huntingtin, and sterol regulatory element binding proteins (SREBPs). CASP3 plays a key role in cell adhesion in sympathetic neurons through RET cleavage.

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  • Sku: PSB-enz-1106-1µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Caspase 3 Apoptosis-Related Cysteine Peptidase, Sf9 is a member of the caspase family of cysteine proteases that play a key role in the initiation and execution of apoptosis. This protein is expressed in the Sf9 insect cell line and has a molecular weight of approximately 32 kDa. Recombinant Human Caspase 3 Apoptosis-Related Cysteine Peptidase, Sf9 is commonly used in research studies to investigate the mechanisms of programmed cell death.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Sf9 - Baculovirus cells
Purity 90% by SDS-PAGE
Activity
 Specific activity is  greater than 5,000 pmol/min/ug. One unit will liberate 1 pmoles of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25C.
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 29.4 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation CASP3 protein solution (0.5mg/ml) containing 20mM HEPES buffer (pH 7.5), 0.1M NaCl, 1mM EDTA, 20% Glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MSGISLDNSY KMDYPEMGLC IIINNKNFHK STGMTSRSGT DVDAANLRET FRNLKYEVRNKNDLTREEIV ELMRDVSKED HSKRSSFVCV LLSHGEEGII FGTNGPVDLK KITNFFRGDRCRSLTGKPKL FIIQACRGTE LDCGIETDSG VDDDMACHKI PVEADFLYAY STAPGYYSWRNSKDGSWFIQ SLCAMLKQYA DKLEFMHILT RVNRKVATEF ESFSFDATFH AKKQIPCIVS MLTKELYFYH HHHHHH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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CASP3, Sf9
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