CAS9

Alternative Names: cas9, csn1, SpyCas9, CRISPR-associated endonuclease Cas9/Csn1

Cas9 is an enzyme found in bacteria, specifically Streptococcus pyogenes, that is associated with the CRISPR adaptive immunity system. It is used by S. pyogenes to recognize and cut foreign DNA. Cas9 can be guided by RNA to target specific sequences of DNA and induce double-strand breaks. This tool has been widely used in genetic engineering to modify genes in various model organisms.

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  • Sku: PSB-enz-901-50µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant S. Pyogenes CRISPR-Associated Protein-9 Nuclease is a type II CRISPR-associated protein derived from Streptococcus pyogenes. Belonging to the Cas9 family of nucleases, it is widely used in genome editing and gene regulation applications. With a molecular weight of approximately 160 kDa, this nuclease is known for its precise and efficient DNA cleavage abilities. Its versatility and specificity make it a valuable tool in molecular biology research and biotechnology.

Current Lead Time

7-14 Days

Product Specifications

Species
Bacteria
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
163 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CAS9

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