CARD18

Alternative Names: Caspase recruitment domain family member 18, Caspase-1 inhibitor Iceberg, ICEBERG caspase-1 inhibitor, ICEBERG, pseudo-ICE, UNQ5804.

CARD18 is a member of the death-domain-fold superfamily that is activated by proinflammatory signals. It primarily consists of a Caspase recruitment domain and functions to suppress the production of IL-1b. This is achieved by cooperating with caspase-1 and preventing its interaction with RIP 2. The interaction between CARD18 and caspase-1 is facilitated by charge-charge interactions between the prodomain of caspase-1 and the surface charge of CARD18.

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  • Sku: PSB-pro-1061-2µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human Caspase Recruitment Domain Family, Member 18 (CARD18) is a protein belonging to the caspase recruitment domain (CARD) family. With a molecular weight of approximately X kDa, CARD18 plays a role in the regulation of apoptotic cell death and immune signaling pathways. This protein is involved in the activation of caspases, which are crucial mediators of programmed cell death. Understanding the functions and mechanisms of CARD18 can provide valuable insights into the intricate processes governing cell survival and immune responses.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
12.3 kDa
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The LSM3 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL VTGKGPKSCC KFIKHLCEED PQLASKMGLH

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CARD18

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