CA3 His

Alternative Names: Carbonic anhydrase III, Carbonic anhydrase 3, Carbonate dehydratase III, CAIII, CA-III, EC 4.2.1.1.

Carbonic anhydrase is a group of metalloenzymes that speed up the reaction converting carbon dioxide to bicarbonate and protons. This reaction would normally occur slowly without this enzyme. Most carbonic anhydrases have a zinc ion in their active site. One type, CAIII, is normally found in the cytoplasm but can be released into the bloodstream after an injury.

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  • Sku: PSB-enz-270-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Carbonic Anhydrase III, His Tag is a member of the carbonic anhydrase enzyme family, which plays a critical role in catalyzing the reversible hydration of carbon dioxide. This specific protein has a molecular weight of approximately 29 kDa and is commonly used in research and biotechnology applications for its ability to efficiently catalyze the interconversion of carbon dioxide and bicarbonate ions. The addition of the His tag allows for easy purification and detection of the protein in various experimental settings.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Supplied in 10mM Tris-HCl (pH 8), 250mM NaCl, 0.5mM DTT, 1.5mM Cysteine, and 50% Glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CA3 His

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