CA1, E.coli

Alternative Names: Carbonate dehydratase, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, CAN, ECK0125, JW0122, yadF, CA 1, CA I, CA1, CAI, Car 1, Car1.

Carbonic anhydrase is a Zn-containing enzyme that facilitates the conversion of CO2 and H2O to HCO3- and H+. This enzyme plays a crucial role in maintaining acid-base balance in the blood and organs. It also assists Carbonic Anhydrase I in transporting CO2 to and from tissues.

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  • Sku: PSB-enz-1138-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant E.Coli Carbonic Anhydrase-1 (CA-1) is a protein enzyme derived from Escherichia coli bacteria through genetic engineering techniques. Belonging to the carbonic anhydrase family, CA-1 plays a significant role in catalyzing the reversible hydration of carbon dioxide, thereby facilitating the interconversion of carbon dioxide and bicarbonate ions. With a molecular weight of approximately X kilodaltons, this enzyme serves as a vital catalyst in various biological processes, including pH regulation, ion transport, and CO2 homeostasis. Its robust enzymatic activity and structural stability make Recombinant E.Coli Carbonic Anhydrase-1 an essential tool in numerous biotechnological and biomedical applications.

Current Lead Time

7-14 Days

Product Specifications

Species
E.Coli
Expression System E. coli
Purity 95.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation CA1 E.Coli protein (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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CA1, E.coli

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