C9

Alternative Names: Not Available

C9 binds to the C5b-8 complex, forming the mature membrane attack complex on cell membranes. Proteolytic enzyme complexes are generated by each pathway of complement activation, which then bind to the target surface. These enzymes cleave a peptide bond in the larger alpha chain of C5, releasing the anaphylatoxin C5a and activating C5b. This is the only step in the assembly of the C5b-9 complex that involves proteolysis. C5b, despite being unstable, remains bound to the activating complex for a short period. During this time, it either binds a single C6 from the surrounding fluid or decays and loses the ability to form the membrane attack complex. The C5b,6 complex can also stay connected to the C3/C5 convertase. Binding a single C7 exposes a membrane-binding region, enabling C5b,6,7 to enter the target cell's bilipid layer. Each C5b-7 complex can bind 1 C8 molecule, leading to a stronger incorporation into the membrane. The C5b-8 complex can cause lysis without C9, albeit at a slower rate and requiring more complexes per cell. The primary role of C8 is to facilitate the binding of C9. Each C8 molecule can bind another C9, initiating the formation of a ring structure with up to 18 C9 molecules. The complexes containing 1 or more C9 molecules are referred

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  • Sku: PSB-pro-2696-20µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Human Complement C9 is a vital component of the complement system, a complex network of proteins that plays a crucial role in the innate immune response. Belonging to the family of complement components, C9 is a glycoprotein with a molecular weight of approximately 63 kilodaltons. It is primarily synthesized in the liver and circulates in the bloodstream as an inactive precursor. Upon activation, C9 assembles into polymeric structures known as the membrane attack complex (MAC), which forms pores in the membranes of target cells, leading to their lysis. The understanding of Human Complement C9 and its functional properties provides valuable insights into the immune defense mechanisms and its potential therapeutic applications.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Human Plasma
Purity 95.0 by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation C9 protein 1mg/ml solution containsPBS pH 7.2.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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C9
SUBHEADING

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