C4

Alternative Names: Acidic complement C4, Complement C4-A, C4A, CO4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, CPAMD2.

Native human C4 is an unglycosylated polypeptide with 3 disulfide-linked chains. It plays a crucial role in activating both the lectin and classical pathways of complement activation. Activation of each pathway leads to the formation of proteolytic enzyme complexes that bind to the target surface. These enzymes cleave a peptide bond in C4, resulting in the release of anaphylatoxin C4a and activation of C4b. Similar to C3, the reactive thioester in metastable C4b can covalently bind to amino or hydroxyl groups on the target surface. In humans, there are two variants of C4, C4A and C4B, with C4B being more common. Other animals typically have only one type of C4.

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  • Sku: PSB-pro-2688-20µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Human Complement C4 is a protein component of the complement system, a crucial part of the immune response. Belonging to the C4 family, it plays a significant role in the activation of the classical pathway of complement activation. With a weight of approximately 190 kDa, Human Complement C4 acts as a precursor to C4a and C4b, which mediate various immune functions, including opsonization and lysis of pathogens. Understanding the structural and functional aspects of Human Complement C4 is vital for comprehending the intricate mechanisms of the immune system.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System Human Plasma
Purity 90.0 by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation C4 protein solution contains 10mM sodium phosphate and 145mM NaCl, pH 7.3.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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C4

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