C3

Alternative Names: Complement C3, C3, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

Native human C3 is a glycosylated polypeptide with two disulfide-linked chains. C3 plays a central role in activating all three pathways of complement activation. Each pathway initiates the formation of proteolytic enzyme complexes that bind to the target surface. These enzymes cleave a peptide bond in C3, resulting in the release of C3a (an anaphylatoxin) and activation of C3b. However, most activated C3 does not bind to the surface. Instead, it reacts with water to form fluid phase C3b, which is rapidly inactivated by factors H and I to form iC3b. Surface-bound C3b is essential in all three pathways for efficient activation of C5 and the formation of C5b-9 complexes, which ultimately lead to the lysis of the target cell membrane.

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  • Sku: PSB-pro-2684-20µg
  • Vendor: ProSpec-Tany
$220.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Human Complement C3 is a key protein involved in the complement system, an integral part of the immune response. Belonging to the family of complement components, C3 plays a crucial role in the activation of the complement cascade. With a weight of approximately 185 kDa, this protein is synthesized in the liver and circulates in the bloodstream. Upon activation, C3 undergoes proteolytic cleavage, generating biologically active fragments that mediate various immune functions, including opsonization, inflammation, and immune cell recruitment. Understanding the intricate mechanisms of C3 activation and regulation is of great significance in elucidating immune-related disorders and developing therapeutic interventions.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System Human Plasma
Purity 94.0 by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation C3 solution contains phosphate buffer saline.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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C3
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