bThrombin

Alternative Names: Coagulation Factor II (Thrombin), Prepro-Coagulation Factor II, Prothrombin B-Chain, Serine Protease, Prothrombin, EC 3.4.21, PT, F2, EC 3.4.21.5, RPRGL2, THPH1.

Thrombin is a key enzyme responsible for blood clotting. It promotes the transformation of soluble fibrinogen into insoluble fibrin, which is essential for clot formation. Thrombin acts as a coagulation protein and a serine protease, catalyzing various reactions related to clotting. It triggers the activation of factors XI, V, XIII, and VIII. Thrombin also activates platelets by interacting with specific receptors on their surface. Due to its high specificity, thrombin is widely used in biochemistry. In particular, the cleavage site of thrombin (Leu-Val-Pro-Arg-Gly-Ser) is commonly employed in recombinant fusion protein constructs to remove purification tags. After the fusion protein is purified, thrombin is used to cleave the site, resulting in the specific removal of the purification tag.

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  • Sku: PSB-pro-447-2000U
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Bovine Thrombin is a protein derived from the blood of cattle that plays a vital role in the coagulation cascade. Belonging to the serine protease family, this enzyme is responsible for converting fibrinogen into fibrin, thereby promoting blood clot formation. With a molecular weight of approximately 36 kDa, Bovine Thrombin acts as a catalyst in the final step of clot formation, ensuring hemostasis during wound healing or surgical procedures. Its precise mechanism and reliable efficacy make Bovine Thrombin a widely used tool in various medical applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Cattle
Expression System Bovine Blood
Purity
Activity
164 US unites/mg protein.
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Monomer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Lyophilized freeze dry powder formulated with glycine, CaCl2 and sodium chloride, PH 6.8.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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bThrombin

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