BPHL

Alternative Names: Biphenyl Hydrolase-Like (serine hydrolase), Bph-rp, Breast Epithelial Mucin-Associated Antigen, MCNAA, VACVASE, MGC41865, MGC125930.

BPHL is a Serine hydrolase from the AB hydrolase superfamily that activates amino acid ester prodrugs of nucleoside analogs. It is primarily found in large quantities in the liver and kidney, with smaller amounts present in the heart, intestine, and skeletal muscle. BPHL is involved in detoxification processes and specifically targets alpha-amino acid esters with small, hydrophobic, and aromatic side chains, with a preference for a primary alcohol leaving group.

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  • Sku: PSB-enz-055-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Biphenyl Hydrolase-Like is a member of the hydrolase enzyme family, responsible for catalyzing the hydrolysis of biphenyl compounds. With a molecular weight of approximately 30 kDa, this enzyme plays a crucial role in the breakdown of biphenyls in various biological processes. Recombinant Human Biphenyl Hydrolase-Like is commonly used in research and industrial applications for its ability to efficiently metabolize biphenyl compounds.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 31.1 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The BPHL protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)1mM DTT, 50mM NaCl and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSVTSAKVAV NGVQLHYQQT GEGDHAVLLL PGMLGSGETD FGPQLKNLNK KLFTVVAWDP RGYGHSRPPD RDFPADFFER DAKDAVDLMK ALKFKKVSLL GWSDGGITAL IAAAKYPSYI HKMVIWGANA YVTDEDSMIY EGIRDVSKWS ERTRKPLEAL YGYDYFARTC EKWVDGIRQF KHLPDGNICR HLLPRVQCPA LIVHGEKDPL VPRFHADFIH KHVKGSRLHL MPEGKHNLHL RFADEFNKLA EDFLQ

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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BPHL
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