BPGM

Alternative Names: EC 5.4.2.4, Bisphosphoglycerate mutase, BPGM, 2,3-bisphosphoglycerate mutase erythrocyte, 2,3-bisphosphoglycerate synthase, BPG-dependent PGAM.

BPGM, a multifunctional enzyme found in high concentrations in red blood cells, plays a role in decreasing the oxygen affinity of hemoglobin. Deficiency of PGM leads to increased oxygen affinity in cells. BPGM catalyzes 2,3-DPG synthesis as a synthetase and 2,3-DPG degradation as a phosphatase. Mutations in BPGM can result in hemolytic anemia. The enzyme also exhibits phosphoglycerate phosphomutase activity, catalyzing the EC 5.4.2.1 and EC 3.1.3.13 reactions at a reduced rate.

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  • Sku: PSB-enz-505-5µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human 2,3-Bisphosphoglycerate Mutase is an enzyme that belongs to the mutase family. It plays a key role in the regulation of oxygen delivery in red blood cells by catalyzing the conversion of 1,3-bisphosphoglycerate to 2,3-bisphosphoglycerate. This enzyme has a molecular weight of approximately 28 kDa and is crucial for maintaining proper oxygen affinity in hemoglobin. Its activity is essential for normal oxygen transport in the body.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The BPGM solution (0.5mg/ml) contains 20mM Tris-HCl (pH 8.0), 1mM DTT and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNERHYGALIGLNR EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED QGKVKQAKKL EHHHHHH.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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BPGM

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