bNucleosome

Alternative Names: Not Available

Nucleosome core proteins are essential for DNA packaging in eukaryotes. Each core consists of an octamer comprised of two copies of histones 2A, 2B, 3, and 4. These histones surround and wrap around an approximately 140 bp segment of DNA, creating two turns.

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  • Sku: PSB-pro-2557-50µg
  • Vendor: ProSpec-Tany
$300.00 USD
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Title

Product Details

Product Description

The Bovine Nucleosome is a fundamental component of chromatin structure in bovine cells. It belongs to the nucleosome family, which is a complex of DNA and histone proteins that play a crucial role in DNA packaging and gene regulation. With a weight of approximately 200 kilodaltons, the Bovine Nucleosome consists of an octamer of histone proteins (H2A, H2B, H3, and H4) around which 147 base pairs of DNA are wrapped in a highly organized manner. This intricate arrangement enables the compaction of DNA and facilitates various cellular processes, including transcription, replication, and repair.

Current Lead Time

7-14 Days

Product Specifications

Species
cow
Expression System Bovine tissues
Purity 95.0 % by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) No
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Nucleosome Bovine is supplied in 20mM HEPES buffer pH-7.5, 500mM NaCl, 1mM NaCl and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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bNucleosome

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