Benzonase Nuclease, 90%

Alternative Names: Not Available

Serratia marcescens produces a highly active endonuclease that is capable of breaking down nucleic acids in its surrounding environment. This endonuclease, known as Benzonase Nuclease, is often used to remove nucleic acid contamination from purified proteins and assist in downstream processing by reducing viscosity. It is the release of the S. marcescens nuclease that causes the presence of nucleic acid contaminants, as it acts as a "killer gene" leading to the destruction of microorganisms.

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  • Sku: PSB-enz-1150-5000U
  • Vendor: ProSpec-Tany
$70.00 USD
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Title

Product Details

Product Description

Recombinant Serratia Marcescens Benzonase Nuclease, 90% is a highly purified enzyme widely used in molecular biology applications for its ability to efficiently degrade nucleic acids. Belonging to the Serratia nuclease family, this enzyme exhibits a molecular weight of approximately [insert weight]. Its exceptional purity and robust activity make it a valuable tool in various research fields, including DNA and RNA isolation, recombinant protein production, and gene expression analysis.

Current Lead Time

7-14 Days

Product Specifications

Species
bacteria
Expression System E. coli
Purity 90 by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation The Benzonase Nuclease solution contains 50% glycerol, 50 mM Tris-HCl pH 8.0, 20 mM NaCl and 2 mM MgCl2.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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Benzonase Nuclease, 90%

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