bEnterokinase His

Alternative Names: Enterokinase, Enteropeptidase, EC 3.4.21.9, Serine protease 7, ENTK, MGC133046.

Enteropeptidase is a key enzyme in human digestion that is produced by cells in the duodenum wall and secreted from the crypts of Lieberk?hn when food enters the duodenum. It activates trypsinogen to trypsin, which in turn activates other pancreatic digestive enzymes. Enteropeptidase is classified as a serine protease enzyme (EC3.4.21.9) and belongs to the Chymotrypsin-clan of serine proteases, sharing structural similarities with other proteins in this group.

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  • Sku: PSB-enz-655-20IU
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Bovine Enteropeptidase/Enterokinase His Tag is a member of the serine protease family, specifically functioning as a protease enzyme in the digestive system. With a molecular weight of approximately 48 kDa, this enzyme plays a vital role in the cleavage of fusion proteins at the recognition site of the enterokinase enzyme. The His Tag allows for easy purification and identification of the enzyme, making it a valuable tool in various research and biotechnological applications.

Current Lead Time

7-14 Days

Product Specifications

Species
Bovine
Expression System E. coli
Purity
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
28.0 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation Bovine EK is supplied in 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

Check Uniprot Link

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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bEnterokinase His

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