BECN1

Alternative Names: ATG6, beclin1, VPS30, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, GT197.

BECN1, which is part of the beclin family, plays a crucial role in autophagy and antiviral host defense. It works in conjunction with its partner, class III phosphoinositide 3-kinase (PI3K), also known as Vps34, to initiate the formation of the autophagasome during autophagy. Additionally, BECN1 is necessary for the abscission stage of cytokinesis.

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  • Sku: PSB-pro-1289-2µg
  • Vendor: ProSpec-Tany
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Title

Product Details

Product Description

Recombinant Human Beclin 1, Autophagy Related is a protein that plays a significant role in the process of autophagy, which is the cellular mechanism responsible for recycling and eliminating damaged or unnecessary cellular components. Belonging to the Beclin family, this protein is crucial for the initiation of autophagy and is involved in the formation of autophagosomes. With a weight of approximately X kDa, Recombinant Human Beclin 1, Autophagy Related serves as a key regulator in maintaining cellular homeostasis and is involved in various physiological and pathological processes.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 85.0% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
54.1 kDa
Structure Dimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation BECN1 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.5), 0.1M NaCl, 10% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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BECN1

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