BAG3

Alternative Names: BIS, CAIR-1, BAG-3, BAG3, BAG Family Molecular Chaperone Regulator 3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1, MGC104307.

BAG3 is a protein that inhibits the chaperone activity of HSP70/HSC70 by promoting the release of substrates. It has anti-apoptotic properties. BAG proteins, including BAG3, interact with Hip to bind to the ATPase domain of Hsc70/Hsp70 and facilitate substrate release. BAG proteins have a BAG domain close to the C terminus, but they differ in their N-terminal regions. In the case of BAG3, it has a WW domain in the N-terminal region and a BAG domain in the C-terminal region. In vitro and in mammalian cells, the BAG domains of BAG1, BAG2, and BAG3 specifically bind with high affinity to the Hsc70 ATPase domain and inhibit its chaperone activity. BAG3 acts as a protein-refolding cochaperone of the bcl2 binding protein BAG family and is upregulated in response to persistent cellular calcium imbalance. It has been shown to reduce stress-induced apoptosis.

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  • Sku: PSB-pro-760-2µg
  • Vendor: ProSpec-Tany
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Product Details

Product Description

Recombinant Human BCL2-Associated Athanogene 3 (BAG3) is a protein that belongs to the BAG family. With a molecular weight of approximately 75 kilodaltons, BAG3 plays a significant role in various cellular processes. This protein is involved in chaperone-mediated protein folding, protein degradation, and cell survival pathways. BAG3 has been extensively studied due to its implications in several diseases, including cancer and neurodegenerative disorders. Understanding the molecular mechanisms and functions of BAG3 is crucial for advancing our knowledge in these areas.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The BAG3 protein contains 20mM Tris buffer pH-8, 1mM EDTA, 10% glycerol and 0.1mM PMSF.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSAATHSPMM QVASGNGDRD PLPPGWEIKI DPQTGWPFFV DHNSRTTTWN DPRVPSEGPK ETPSSANGPS REGSRLPPAR EGHPVYPQLR PGYIPIPVLH EGAENRQVHP FHVYPQPGMQ RFRTEAAAAA PQRSQSPLRG MPETTQPDKQ CGQVAAAAAA QPPASHGPER SQSPAASDCS SSSSSASLPS SGRSSLGSHQ LPRGYISIPV IHEQNVTRPA AQPSFHQAQK THYPAQQGEY QTHQPVYHKI QGDDWEPRPL RAASPFRSSV QGASSREGSP ARSSTPLHSP SPIRVHTVVD RPQQPMTHRE TAPVSQPENK PESKPGPVGP ELPPGHIPIQ VIRKEVDSKP VSQKPPPPSE KVEVKVPPAP VPCPPPSPGP SAVPSSPKSV ATEERAAPST APAEATPPKP GEAEAPPKHP GVLKVEAILE KVQGLEQAVD NFEGKKTDKK YLMIEEYLTK ELLALDSVDP EGRADVRQAR RDGVRKVQTI LEKLEQKAID VPGQVQVYEL QPSNLEADQP LQAIMEMGAV AADKGKKNAG NAEDPHTETQ QPEATAAATS NPSSMTDTPG NPAAP.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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BAG3
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