AURKA

Alternative Names: Aurora kinase A, Aurora-A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, AURKA, AIK, ARK1, AURA, BTAK, Serine/threonine-protein kinase 6, STK6, STK7, Serine/threonine kinase 15, STK15, Breast tumor-amplified kinase, hARK1, MGC34538, AURORA2.

AURKA, a member of the mitotic serine/threonine kinases family, plays a crucial role in cell cycle regulation by assisting in microtubule formation/stabilization at the spindle pole during chromosome segregation. It is predominantly located at the centrosome in interphase cells and at the spindle poles in mitosis. AURKA expression fluctuates throughout the cell cycle, peaking during G2/M phase and decreasing rapidly afterwards. Its proper function is vital for healthy cell proliferation during mitosis and meiosis. AURKA is overexpressed in various cancers such as colorectal, ovarian, prostate, neuroblastoma, breast, and cervical cancers, as well as testis. It interacts with key proteins like BORA, ARHGEF2, TACC1, and CPEB1. Abnormalities in the AURKA gene can lead to numerical centrosome aberrations, potentially causing chromosomal instability and aneuploidy. Its expression levels may serve as a prognostic indicator in ovarian carcinoma.

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  • Sku: PSB-pka-350-2µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human Aurora Kinase A is a protein belonging to the serine/threonine kinase family. It plays a crucial role in cell cycle regulation and mitosis. With a molecular weight of approximately 46 kDa, Aurora Kinase A is involved in the phosphorylation of various substrates that are essential for proper cell division. Its dysregulation has been linked to cancer development and progression, making it a potential target for anti-cancer therapies.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 47.9 kDa
Structure Dimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The AURKA solution containing 20mM Tris-HCl buffer (pH 8.0), 0.5mM DTT, 100mM NaCl, 0.1mM EDTA, 0.1mM EGTA, 0.1mM PMSF and 20% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRIPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILALKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKRISRVEFTFPD FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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AURKA
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