AUH

Alternative Names: Methylglutaconyl-CoA hydratase, mitochondrial, AU-specific RNA-binding protein/enoyl-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AUH

Mitochondrial methylglutaconyl-CoA hydratase (AUH) plays a key role in the amino acid degradation pathway by converting 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and water. The human AUH gene produces a 1.8 kb mRNA that is translated into a 40kDa precursor protein, eventually processed into a 32kDa mature form. AUH exhibits low enoyl-CoA hydratase activity and binds to AU-rich elements (ARE) found in rapidly decaying mRNA, such as c-fos, c-myc, and granulocyte/macrophage colony stimulating factor. These elements are responsible for directing RNA to degradation and deadenylation. AUH is also similar to enol-CoA hydratase, which is involved in fatty acid degradation, demonstrating its dual role as an RNA binding protein and metabolic enzyme.

  •
  • Sku: PSB-enz-046-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Safety Data Sheet
UniProt Listing
NCBI Link

Product Details

Product Description

Recombinant Human AU RNA Binding Protein/Enoyl-CoA Hydratase is a multifunctional protein that plays a critical role in RNA metabolism and lipid metabolism. Belonging to the RNA-binding protein family, this protein has a molecular weight of approximately 80 kDa. Its dual functionality as both an RNA binding protein and an enoyl-CoA hydratase makes it a versatile player in cellular processes. This recombinant protein is commonly used in research settings to study its various functions and interactions within the cell.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The AUH solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSSEMKTEDE LRVRHLEEEN RGIVVLGINR AYGKNSLSKN LIKMLSKAVD ALKSDKKVRT IIIRSEVPGI FCAGADLKER AKMSSSEVGP FVSKIRAVIN DIANLPVPTI AAIDGLALGG GLELALACDI RVAASSAKMG LVETKLAIIP GGGGTQRLPR AIGMSLAKEL IFSARVLDGK EAKAVGLISH VLEQNQEGDA AYRKALDLAR EFLPQGPVAM RVAKLAINQG MEVDLVTGLA IEEACYAQTI PTKDRLEGLL AFKEKRPPRY KGE.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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AUH
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