ATPIF1

Alternative Names: ATPase inhibitor mitochondrial, Inhibitor of F(1)F(o)-ATPase, ATPIF1, IF(1), IF1, ATPI, IP, ATPIP.

ATPase inhibitory factor 1 (ATPIF1) regulates the activity of F1-ATPase, which is responsible for energy production in cells. ATPIF1 attaches to a specific region of the F1-ATPase and interferes with the interaction between its beta and gamma subunits. This control mechanism is particularly relevant at low pH levels. In addition to this role, ATPIF1 is involved in the development of cancer. Overexpression of ATPIF1 in human carcinomas contributes to oncogenesis and provides insight into the altered energy metabolism observed in cancer cells. ATPIF1 also acts as an endogenous inhibitor of F1F(o)-ATPase, preventing excessive depletion of ATP when the mitochondrial membrane potential decreases below a certain threshold. This happens when the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.

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  • Sku: PSB-pro-1154-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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UniProt Listing
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Product Details

Product Description

Recombinant Human ATPase Inhibitory Factor 1 (ATPIF1) is a protein that belongs to the ATPase inhibitory factor family. With a molecular weight of approximately X kDa, ATPIF1 plays a significant role in regulating ATP synthase activity by binding to the F1 sector and inhibiting its ATP hydrolysis. This inhibition prevents the reversal of ATP synthase, thereby maintaining cellular energy homeostasis. ATPIF1's molecular properties and functional mechanisms make it a valuable target for studying ATP synthesis and energy metabolism in various biological systems.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 95% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 12.2 kDa
Structure Homodimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation ATPIF1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
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References

Not Available
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ATPIF1
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