ATP6V1F

Alternative Names: Not Available

ATPase Transporting, Lysosomal V1 Subunit F (ATP6V1F) is a subunit of vacuolar ATPase (V-ATPase), an enzyme that acidifies organelles in eukaryotic cells. This acidification is necessary for processes like protein sorting and endocytosis. V-ATPase has two domains: V1, which contains the ATP catalytic site, and V0, made up of subunits a, c, c', c', and d. The V1 domain includes subunits A, B, G, C, D, E, F, and H.

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  • Sku: PSB-pro-2166-5µg
  • Vendor: ProSpec-Tany
$60.00 USD
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Title

Product Details

Product Description

Recombinant Human ATPase Transporting, Lysosomal V1 Subunit F, also known as ATP6V1F, is a protein that belongs to the ATPases associated with various cellular activities (AAA+) family. This protein plays a significant role in the acidification of intracellular compartments, particularly lysosomes. With a molecular weight of approximately X kilodaltons, ATP6V1F is involved in the transport of protons across the lysosomal membrane, contributing to the maintenance of proper cellular pH levels. Its functional importance in cellular processes makes ATP6V1F a subject of interest in various research fields, including cell biology and biochemistry.

Current Lead Time

7-14 Days

Product Specifications

Species
Human
Expression System E. coli
Purity 90% by SDS-PAGE
Activity
Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
N/A
Structure Homodimer
Endotoxin Concentration
Not Available
Purification Method Not Available
Form
Frozen
Formulation ATP6V1F protein solution (0.5mg/ml) containing Phosphate buffered saline (pH7.4), 50% glycerol and 1mM DTT.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAGRGKL IAVIGDEDTV TGFLLGGIGE LNKNRHPNFL VVEKDTTINE IEDTFRQFLN RDDIGIILIN QYIAEMVRHA LDAHQQSIPA VLEIPSKEHP YDAAKDSILR RARGMFTAED LR.

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution

References

Not Available

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ATP6V1F

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