ATP5F1

Alternative Names: ATP Synthase B Chain Mitochondrial, ATP Synthase H+ Transporting, Mitochondrial Fo Complex Subunit B Isoform 1, ATP Synthase Proton-Transporting Mitochondrial F(0) Complex Subunit B1, ATPase Subunit B, Cell Proliferation-Inducing Protein 47, PIG47.

ATP5F1 is a subunit of mitochondrial ATP synthase that plays a role in ATP synthesis. It uses an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two complexes: F1, which is the catalytic core, and Fo, which is the membrane-spanning component and includes the proton channel. The catalytic part of mitochondrial ATP synthase consists of 9 subunits: 3 alpha, 3 beta, and 1 each of gamma, delta, and epsilon. The proton channel is made up of 9 subunits (a, b, c, d, e, f, g, F6, and 8).

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  • Sku: PSB-pro-1839-5µg
  • Vendor: ProSpec-Tany
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Safety Data Sheet
UniProt Listing
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Product Details

Product Description

Recombinant Human Synthase Transporting Mitochondrial Fo Complex B1, a protein encoded by the ATP5F1B gene, is a crucial component of the mitochondrial F1F0-ATP synthase complex. Belonging to the ATP synthase family, this protein plays a vital role in the synthesis of ATP, the main energy currency of the cell. With a molecular weight of approximately 17 kDa, Recombinant Human Synthase Transporting Mitochondrial Fo Complex B1 is involved in the translocation of protons across the mitochondrial inner membrane, facilitating ATP production. Its significance lies in its contribution to cellular energy metabolism and overall mitochondrial function.

Current Lead Time

7-14 Days

Product Specifications

Species
 Human
Expression System E. coli
Purity 80% by SDS-PAGE
Activity
 Not Available
Activity Assay
Animal Component Free (ACF) Yes
Molecular Weight
 N/A
Structure Homomultimer
Endotoxin Concentration
 Not Available
Purification Method Not Available
Form
 Frozen
Formulation The ATP5F1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

*For Research Use Only

Product Guarantee

If your product is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM

Storage Instruction

Reconstituted store at -20°C or -80°C.We recommend that single use aliquots are prepared to avoid freeze-thaw cycles.

Sample Prep Instruction

1. Thaw the vial on ice, spin briefly
2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
4. We recommend sterile filtering after dilution in media or the final working solution
​

References

Not Available
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ATP5F1
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